A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes

Clicks: 334
ID: 271390
1993
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Abstract
We have developed a simple non-radioactive in situ hybridization procedure for tissue sections and cultured cells using digoxigenin-labelled cRNA probes. This protocol can be applied for the detection of various transcripts present at a wide range of expression levels in the central nervous system. …
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Authors Schaeren-Wiemers N;Gerfin-Moser A;;
Journal Histochemistry
Year 1993
DOI
DOI not found
URL
http://www.ncbi.nlm.nih.gov/pubmed/7512949
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE RNA animals pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't Cells Cultured Rats genetic* Receptors Messenger / genetics transcription messenger / metabolism immunohistochemistry ethanol cerebellum / metabolism octoxynol pmid:7512949 doi:10.1007/bf00267823 n schaeren-wiemers a gerfin-moser bisbenzimidazole digoxigenin glial fibrillary acidic protein / metabolism in situ hybridization* inositol polyphosphate 5-phosphatases myelin basic protein / genetics myelin basic protein / metabolism nerve tissue / metabolism* phosphoric monoester hydrolases / genetics phosphoric monoester hydrolases / metabolism complementary* glutamate / genetics glutamate / metabolism

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