Lecithin is the key material attribute in soy bean oil affecting filamentous bioprocesses

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ID: 27069
2018
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Abstract
Abstract Complex raw materials are widely used as supplements in biopharmaceutical production processes due to their positive effect on biomass growth and productivity at low cost. However, their use negatively impacts process reproducibility due to high lot-to-lot variability which contradicts current regulatory guidelines. In this study we investigated crude soy bean oil (SBO) which is a common complex raw material for filamentous fungi. We demonstrated that lecithin, which we define as phosphatidylcholines, is in fact the key material attribute in crude SBO positively affecting fungal growth and consequently productivity. The methodological toolbox we present here allows the straightforward isolation of lecithin from crude SBO, its semi-quantification by HPLC and the consequent supplementation thereof in defined amounts. Thus, over-dosage and potential resulting negative impacts on fungal growth and productivity can be omitted.
Reference Key
hofer2018lecithinamb Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Hofer, Alexandra;Herwig, Christoph;Spadiut, Oliver;
Journal amb express
Year 2018
DOI
DOI not found
URL
http://link.springer.com/article/10.1186/s13568-018-0625-0
Keywords Keywords not found

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