Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum cytophaga-flavobacter-bacteroides in the natural environment

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ID: 270213
1996
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Abstract
We designed a panel of four 16S rRNA-targeted oligonucleotide probes specific for bacteria of the phylum cytophaga-flavobacter-bacteroides (CFB). Probes CF319a and CF319b are targeted to members of the flavobacteria-cytophaga group and the genus Porphyromonas, whereas probe BAC303 has a target region characteristic for the genera Prevotella and Bacteroides within the bacteroides group. The probe FFE8b was developed for species-specific hybridizations with Flavobacterium ferrugineum. All probes were designed by computer-assisted sequence analysis and compared to all currently accessible 16S and 23S rRNA sequences. The oligonucleotides were further evaluated by whole-cell and non-radioactive dot-blot hybridization against reference strains of the CFB phylum and other major lineages of Bacteria. The newly developed probes were used together with other higher-order probes to analyse the structure and community composition in complex environments. In activated sludge samples, members of the flavobacteria-cytophaga group were revealed by in situ hybridization as important constituents of sludge flocs and characteristic colonizers of filamentous bacteria. By application of fluorescent probe BAC303, members of the genera Bacteroides and Prevotella could be visualized without prior cultivation as an important part of the human faecal microflora.
Reference Key
manz1996microbiologyapplication Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Werner Manz;Rudolf Amann;Wolfgang Ludwig;Marc Vancanneyt;Karl-Heinz Schleifer;Werner Manz;Rudolf Amann;Wolfgang Ludwig;Marc Vancanneyt;Karl-Heinz Schleifer;
Journal microbiology
Year 1996
DOI
doi:10.1099/13500872-142-5-1097
URL
http://dx.doi.org/10.1099/13500872-142-5-1097
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE RNA humans pubmed abstract nih national institutes of health national library of medicine research support non-u.s. gov't Comparative Study Ribosomal Oligonucleotide Probes* Base Sequence Molecular Sequence Data Phylogeny feces / microbiology 16s / genetics* bacteroides / genetics environmental microbiology* bacterial / genetics* pmid:8704951 doi:10.1099/13500872-142-5-1097 werner manz rudolf amann karl-heinz schleifer bacteroides / isolation & purification* cytophaga / genetics cytophaga / isolation & purification* flavobacterium / genetics flavobacterium / isolation & purification* gram-negative bacteria / classification*

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