A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas

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ID: 269716
2002
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Abstract
We have characterized a novel nuclease from the Kamchatka crab, designated duplex-specific nuclease (DSN). DSN displays a strong preference for cleaving double-stranded DNA and DNA in DNA-RNA hybrid duplexes, compared to single-stranded DNA. Moreover, the cleavage rate of short, perfectly matched DN …
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da2002genomea Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Shagin DA, Rebrikov DV, Kozhemyako VB, Altshuler IM, Shcheglov AS, Zhulidov PA, Bogdanova EA, Staroverov DB, Rasskazov VA, Lukyanov S
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Journal genome research
Year 2002
DOI
DOI not found
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http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=12466298
Keywords
National Center for Biotechnology Information NCBI NLM MEDLINE genetic animals pubmed abstract nih national institutes of health national library of medicine models research support non-u.s. gov't Molecular Sequence Data Polymorphism endonucleases / genetics* cloning single nucleotide / genetics* liver / enzymology pmid:12466298 pmc187582 doi:10.1101/gr.547002 dmitry a shagin denis v rebrikov sergey lukyanov anomura / enzymology* anomura / genetics* molecular / methods nucleic acid heteroduplexes / genetics* oligonucleotides / chemical synthesis oligonucleotides / metabolism pancreas / enzymology substrate specificity / genetics

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