Enzymatic in situ saccharification of chestnut shell with high ionic liquid-tolerant cellulases from Galactomyces sp. CCZU11-1 in a biocompatible ionic liquid-cellulase media.

Clicks: 331
ID: 2694
2016
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Abstract
In this study, it was the first time to report that the cellulases of Galactomyces sp. CCZU11-1 showed high activity and stability in the culture and reaction media containing IL [Mmim]DMP. Using untreated chestnut shell (CNS) as carbon source in the culture media containing IL [Mmim]DMP (5%, w/v), high activity of FPA (28.6U/mL), xylanase (186.2U/mL), and CMCase (107.3U/mL) were obtained, and 184.9mg/L of total protein was achieved. Furthermore, the changes in the structural features (crystallinity, morphology, and porosity) of the solid residue of CNS utilized with Galactomyces sp. CCZU11-1 were characterized with Fourier transform infrared spectroscopy, scanning electron microscopy, and X-ray diffraction. After was enzymatically hydrolyzed with the prepared crude enzymes in IL diluted to 20% (w/v), a high yield of reducing sugars, 62.1%, was obtained. Significantly, Galactomyces sp. CCZU11-1 showed high potential for the efficient transformation of lignocellulosic materials to glucose in a single-step process.
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he2016enzymaticbioresource Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors He, Yu-Cai;Liu, Feng;Gong, Lei;Di, Jun-Hua;Ding, Yun;Ma, Cui-Luan;Zhang, Dan-Ping;Tao, Zhi-Cheng;Wang, Cheng;Yang, Bin;
Journal Bioresource technology
Year 2016
DOI
10.1016/j.biortech.2015.11.034
URL
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