Application of xCELLigence RTCA Biosensor Technology for Revealing the Profile and Window of Drug Responsiveness in Real Time

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ID: 268931
2015
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Abstract
The xCELLigence technology is a real-time cellular biosensor, which measures the net adhesion of cells to high-density gold electrode arrays printed on custom-designed E-plates. The strength of cellular adhesion is influenced by a myriad of factors that include cell type, cell viability, growth, migration, spreading and proliferation. We therefore hypothesised that xCELLigence biosensor technology would provide a valuable platform for the measurement of drug responses in a multitude of different experimental, clinical or pharmacological contexts. In this manuscript, we demonstrate how xCELLigence technology has been invaluable in the identification of (1) not only if cells respond to a particular drug, but (2) the window of drug responsiveness. The latter aspect is often left to educated guess work in classical end-point assays, whereas biosensor technology reveals the temporal profile of the response in real time, which enables both acute responses and longer term responses to be profiled within the same assay. In our experience, the xCELLigence biosensor technology is suitable for highly targeted drug assessment and also low to medium throughput drug screening, which produces high content temporal data in real time.
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kho2015biosensorsapplication Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Dan Kho;Christa MacDonald;Rebecca Johnson;Charles P. Unsworth;Simon J. O'Carroll;Elyce Du Mez;Catherine E. Angel;E. Scott Graham;Kho, Dan;MacDonald, Christa;Johnson, Rebecca;Unsworth, Charles P.;O'Carroll, Simon J.;Mez, Elyce Du;Angel, Catherine E.;Graham, E. Scott;
Journal Biosensors
Year 2015
DOI
10.3390/bios5020199
URL
https://www.mdpi.com/2079-6374/5/2/199#citedby
Keywords
Cytotoxicity adhesion drugs cell death responsiveness xcelligence cell index

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