New Nanofibers Based on Protein By-Products with Bioactive Potential for Tissue Engineering

Neue Nanofasern auf Basis von Proteinnebenprodukten mit bioaktivem Potenzial für das Tissue Engineering

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ID: 268512
2020
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Abstract
Concentrated collagen hydrolysate (HC10CC), rabbit collagen glue (RCG), and keratin hydrolysate (KH) were investigated in terms of their extraction from mammalian by-products and processing by electrospinning. The electrospun nanofibers were characterized by scanning electron microscopy coupled with the energy dispersive X-ray spectroscopy (SEM/EDS), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC), and indentation tests. The cytotoxicity of the electrospun nanofibers was conducted on L929 fibroblast cells using MTT and LDH assays and cell morphology observations. The electrospun RCG and KH nanofibers morphology showed an average size of nanofibers ranging between 44 and 410 nm, while the electrospun HC10CC nanofibers exhibited higher sizes. The ATR-FTIR spectra performed both on extracted proteins and electrospun nanofibers showed that the triple helix structure of collagen is partially preserved. The results were in agreement with the circular dichroism analysis for protein extracts. Furthermore, the viscoelastic properties of electrospun KH nanofibers were superior to those of electrospun RCG nanofibers. Based on both in vitro quantitative and qualitative analysis, the electrospun nanofibers were not cytotoxic, inducing a healthy cellular response. The results of new electrospun protein-based nanofibers may be useful for further research on bioactive properties of these nanofibers for tissue engineering.
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Authors Maria Râpă;Carmen Gaidău;Laura Mihaela Stefan;Ecaterina Matei;Mihaela Niculescu;Mariana Daniela Berechet;Maria Stanca;Cristina Tablet;Mădălina Tudorache;Raluca Gavrilă;Cristian Predescu;Ruxandra Vidu;Râpă, Maria;Gaidău, Carmen;Stefan, Laura Mihaela;Matei, Ecaterina;Niculescu, Mihaela;Berechet, Mariana Daniela;Stanca, Maria;Tablet, Cristina;Tudorache, Mădălina;Gavrilă, Raluca;Predescu, Cristian;Vidu, Ruxandra;
Journal Materials (Basel, Switzerland)
Year 2020
DOI
10.3390/ma13143149
URL
https://doi.org/10.3390/ma13143149
Keywords
electrospinning triple helix collagen hydrolysate rabbit collagen glue keratin hydrolysate in vitro cytotoxicity

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