Mechanisms of DNA uptake by mammalian cells: fate of exogenously added DNA monitored by the use of fluorescent dyes

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ID: 267858
1982
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Abstract
Coprecipitation of DNA with calcium phosphate is a commonly used method of gene transfer in mammalian cells. We have found that DNA forms a tight complex with Ca Pi and that DNA in this complex is resistant to nucleases present in serum or added externally. Under optimal conditions, virtually all of the recipient mouse Ltk- Aprt- cells take up Ca Pi--DNA complexes, as determined by fluorescent dyes specific for DNA (4',6-diaminilo-2-phenylindol dihydrochloride) or for calcium salts (chlorotetracycline). However, only a small proportion of the cells have detectable CA Pi--DNA complexes in the nucleus. Uptake of the Ca Pi--DNA complexes was highly dependent upon the pH at which the Ca Pi--DNA complexes was formed and upon the concentration of DNA in the complex.
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loyter1982proceedingsmechanisms Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors A Loyter;G A Scangos;F H Ruddle;A Loyter;G A Scangos;F H Ruddle;
Journal proceedings of the national academy of sciences
Year 1982
DOI
10.1073/pnas.79.2.422
URL
https://doi.org/10.1073/pnas.79.2.422
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