Multicenter evaluation of the reproducibility of the proposed antifungal susceptibility testing method for fermentative yeasts of the Antifungal Susceptibility Testing Subcommittee of the European Committee on Antimicrobial Susceptibility Testing (AFST-EUCAST)

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ID: 267796
2003
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Abstract
ObjectiveTo evaluate the intra- and inter-laboratory reproducibility of a new standard for susceptibility testing of fermentative yeasts. This standard is based on the M27-A procedure of the National Committee for Clinical Laboratory Standards (NCCLS), but incorporates several modifications, including spectrophotometric growth-dependent endpoint reading.MethodsNine laboratories participated in the study. Common material lots were used to test six Candida species (one each of C. albicans, C. tropicalis, C. parapsilosis, C. glabrata, C. krusei, and C. lusitaniae), and two quality control strains (C. krusei ATCC6258 and C. parapsilosis ATCC22019). Triplicate testing on three separate days was performed in microtiter format with RPMI–2% glucose, pH 7.0. Flucytosine, fluconazole and itraconazole were tested. In total, 3888 MIC values were included in the analyses. Reproducibility was calculated by means of agreement (percentage of MICs within one two-fold dilution of the mode) and intraclass correlation coefficient (ICC, maximum value of 1).ResultsThe average intra-laboratory agreements were 99% and 96% after 24 h and 48 h of incubation, respectively, with ICCs of 0.98 and 0.97 (P < 0.05). Two strains exhibiting a trailing effect showed intra-laboratory agreement of 92% and ICCs of
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rodríguez-tudela2003clinicalmulticenter Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors M. Cuenca-Estrella,C.B. Moore,F. Barchiesi,J. Bille,E. Chryssanthou,David Denning,J Peter Donnelly,F. Dromer,B. Dupont,John Rex,Malcolm Richardson,B. Sancak,Paul Verweij,J.L. Rodríguez-Tudela;M. Cuenca-Estrella;C.B. Moore;F. Barchiesi;J. Bille;E. Chryssanthou;David Denning;J Peter Donnelly;F. Dromer;B. Dupont;John Rex;Malcolm Richardson;B. Sancak;Paul Verweij;J.L. Rodríguez-Tudela;
Journal clinical microbiology and infection
Year 2003
DOI
10.1046/j.1469-0691.2003.00592.x
URL
https://sciprofiles.com/publication/view/ae63f5583b2b3361b898e2a579101541
Keywords
agreement antifungal itraconazole procedure iccs inter laboratory susceptibility testing eucast nccls

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