Computational Design of a Molecularly Imprinted Polymer for the Biomonitoring of the Organophosphorous Metabolite Chlorferron

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ID: 267738
2021
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Abstract
Coumaphos is an organophosphorus compound used as insecticide and frequently used by beekeepers for the management of parasitic mites. The most important metabolite, chlorferron (CFN), has been identified in biological samples and foodstuff. The need to quickly identify the presence of typical metabolites, as an indication of interaction with coumaphos has driven the need to produce a highly sensitive electrochemical method for chlorferron analysis, based on molecularly imprinting polymers (MIP) technology. It showed irreversible behaviour with mixed diffusion/adsorption-controlled reactions at the electrode surface. A monoelectronic mechanism of reaction for oxidation has also been suggested. The linear range observed was from 0.158 to 75 µM. Median precision in terms of %RSD around 3% was also observed. For DPV, the limit of detection (LOD) and the limit of quantitation (LOQ) for the CFN-MIP were 0.158 µM and 0.48 µM, respectively. The obtained median % recovery was around 98%. The results were also validated to reference values obtained using GC-MS. Urine and human synthetic plasma spiked with CFN were used to demonstrate the usability of the method in biological samples, showing the potential for biomonitoring. The developed imprinted sensor showed maximum signal change less than 16.8% when related metabolites or pesticide were added to the mix, suggesting high selectivity of the MIP sensor toward CFN molecules. The results from in vitro metabolism of CMP analysed also demonstrates the potential for detection and quantification of CFN in environmental samples. The newly developed CFN-MIP sensor offers similar LoDs than chromatographic methods with shorter analysis time.
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qader2021biosensorscomputational Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Bakhtiyar Qader;Issam Hussain;Mark Baron;Rebeca Jiménez-Pérez;Guzmán Gil-Ramírez;Jose Gonzalez-Rodriguez;Qader, Bakhtiyar;Hussain, Issam;Baron, Mark;Jiménez-Pérez, Rebeca;Gil-Ramírez, Guzmán;Gonzalez-Rodriguez, Jose;
Journal Biosensors
Year 2021
DOI
10.3390/bios11060192
URL
https://www.mdpi.com/2079-6374/11/6/192/htm##B11-biosensors-11-00192
Keywords
Biomarker environmental analysis electrochemical detection coumaphos metabolite bee pesticide National Center for Biotechnology Information NCBI NLM MEDLINE humans pubmed abstract nih national institutes of health national library of medicine electrodes adsorption limit of detection polymers pmid:34200646 pmc8227226 doi:10.3390/bios11060192 bakhtiyar qader issam hussain jose gonzalez-rodriguez biological monitoring / methods* electrochemical techniques molecular imprinting molecularly imprinted polymers* umbelliferones / analysis* umbelliferones / chemistry

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