The RNA-Binding Protein Rasputin/G3BP Enhances the Stability and Translation of Its Target mRNAs

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ID: 267397
2020
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Abstract
Summary G3BP RNA-binding proteins are important components of stress granules (SGs). Here, we analyze the role of the Drosophila G3BP Rasputin (RIN) in unstressed cells, where RIN is not SG associated. Immunoprecipitation followed by microarray analysis identifies over 550 mRNAs that copurify with RIN. The mRNAs found in SGs are long and translationally silent. In contrast, we find that RIN-bound mRNAs, which encode core components of the transcription, splicing, and translation machinery, are short, stable, and highly translated. We show that RIN is associated with polysomes and provide evidence for a direct role for RIN and its human homologs in stabilizing and upregulating the translation of their target mRNAs. We propose that when cells are stressed, the resulting incorporation of RIN/G3BPs into SGs sequesters them away from their short target mRNAs. This would downregulate the expression of these transcripts, even though they are not incorporated into stress granules.
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lipshitz2020cellthe Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors John D. Laver,Jimmy Ly,Allison K. Winn,Angelo Karaiskakis,Sichun Lin,Kun Nie,Giulia Benic,Nima Jaberi-Lashkari,Wen Xi Cao,Alireza Khademi,J. Timothy Westwood,Sachdev S. Sidhu,Quaid Morris,Stephane Angers,Craig A. Smibert,Howard D. Lipshitz;John D. Laver;Jimmy Ly;Allison K. Winn;Angelo Karaiskakis;Sichun Lin;Kun Nie;Giulia Benic;Nima Jaberi-Lashkari;Wen Xi Cao;Alireza Khademi;J. Timothy Westwood;Sachdev S. Sidhu;Quaid Morris;Stephane Angers;Craig A. Smibert;Howard D. Lipshitz;
Journal Cell reports
Year 2020
DOI
10.1016/j.celrep.2020.02.066
URL
https://sciprofiles.com/publication/view/830d9f6ccb5a51b81089941af5aeef17
Keywords
rna-binding protein; post-transcriptional regulation; mrna translation; mrna stability; stress granule; drosophila; embryo; g3bp; rasputin

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