Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation

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ID: 267288
2017
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Abstract
Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed lymphocyte culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication.
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revenfeld2017internationalinduction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Anne Louise Schacht Revenfeld;Rikke Bæk;Malene Møller Jørgensen;Kim Varming;Allan Stensballe;Revenfeld, Anne Louise Schacht;Bæk, Rikke;Jørgensen, Malene Møller;Varming, Kim;Stensballe, Allan;
Journal International journal of molecular sciences
Year 2017
DOI
10.3390/ijms18071603
URL
https://www.mdpi.com/1422-0067/18/7/1603/htm##B39-ijms-18-01603
Keywords
flow cytometry regulatory t cells peripheral tolerance ev array National Center for Biotechnology Information NCBI NLM MEDLINE humans pubmed abstract nih national institutes of health national library of medicine female male t-lymphocytes cell culture techniques regulatory / immunology* pmid:28737722 pmc5536089 doi:10.3390/ijms18071603 anne louise schacht revenfeld rikke bæk allan stensballe antigen-presenting cells / cytology antigen-presenting cells / metabolism* cd3 complex / immunology* cell communication / immunology* hla-dr antigens / immunology* lymphocyte activation* regulatory / cytology

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