Induction of a Regulatory Phenotype in CD3+ CD4+ HLA-DR+ T Cells after Allogeneic Mixed Lymphocyte Culture; Indications of Both Contact-Dependent and -Independent Activation

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ID: 267288
2017
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Abstract
Although the observation of major histocompatibility complex II (MHCII) receptors on T cells is longstanding, the explanation for this occurrence remains enigmatic. Reports of an inducible, endogenous expression exist, as do studies demonstrating a protein acquisition from other cells by mechanisms including vesicle transfer. Irrespective of origin, the presence of the human MHCII isotype, human leukocyte antigen DR (HLA-DR), potentially identifies a regulatory T cell population. Using an allogeneic mixed lymphocyte culture (MLC) to induce an antigen-specific immune response, the role of antigen-presenting cells (APCs) for the presence of HLA-DR on cluster of differentiation 3(CD3)+ CD4+ T cells was evaluated. Moreover, a functional phenotype was established for these T cells. It was demonstrated that APCs were essential for HLA-DR on CD3+ CD4+ T cells. Additionally, a regulatory T cell phenotype was induced in CD3+ CD4+ HLA-DR+ responder T cells with an expression of CD25, CTLA-4, CD62L, PD-1, and TNFRII. This phenotype was induced both with and without physical T cell:APC contact, which could reveal novel indications about its functionality. To further investigate contact-independent communication, a phenotype of the small cell-derived vesicles from the MLCs was determined. Yet heterogeneous, this vesicle phenotype displayed contact-dependent differences, providing clues about their intended function in cellular communication.
Reference Key
revenfeld2017internationalinduction Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Anne Louise Schacht Revenfeld;Rikke Bæk;Malene Møller Jørgensen;Kim Varming;Allan Stensballe;Revenfeld, Anne Louise Schacht;Bæk, Rikke;Jørgensen, Malene Møller;Varming, Kim;Stensballe, Allan;
Journal International journal of molecular sciences
Year 2017
DOI
10.3390/ijms18071603
URL
https://www.mdpi.com/1422-0067/18/7/1603/htm##B39-ijms-18-01603
Keywords
flow cytometry regulatory t cells peripheral tolerance ev array National Center for Biotechnology Information NCBI NLM MEDLINE humans pubmed abstract nih national institutes of health national library of medicine female male t-lymphocytes cell culture techniques regulatory / immunology* pmid:28737722 pmc5536089 doi:10.3390/ijms18071603 anne louise schacht revenfeld rikke bæk allan stensballe antigen-presenting cells / cytology antigen-presenting cells / metabolism* cd3 complex / immunology* cell communication / immunology* hla-dr antigens / immunology* lymphocyte activation* regulatory / cytology

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