Investigation of Hemoglobin/Gold Nanoparticle Heterolayer on Micro-Gap for Electrochemical Biosensor Application

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ID: 267017
2016
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Abstract
In the present study, we fabricated a hemoglobin/gold nanoparticle (Hb/GNP) heterolayer immobilized on the Au micro-gap to confirm H2O2 detection with a signal-enhancement effect. The hemoglobin which contained the heme group catalyzed the reduction of H2O2. To facilitate the electron transfer between hemoglobin and Au micro-gap electrode, a gold nanoparticle was introduced. The Au micro-gap electrode that has gap size of 5 µm was fabricated by conventional photolithographic technique to locate working and counter electrodes oppositely in a single chip for the signal sensitivity and reliability. The hemoglobin was self-assembled onto the Au surface via chemical linker 6-mercaptohexanoic acid (6-MHA). Then, the gold nanoparticles were adsorbed onto hemoglobin/6-MHA heterolayers by the layer-by-layer (LbL) method. The fabrication of the Hb/GNP heterolayer was confirmed by atomic force microscopy (AFM) and surface-enhanced Raman spectroscopy (SERS). The redox property and H2O2 detection of Hb/GNP on the micro-gap electrode was investigated by a cyclic voltammetry (CV) experiment. Taken together, the present results show that the electrochemical signal-enhancement effect of a hemoglobin/nanoparticle heterolayer was well confirmed on the micro-scale electrode for biosensor applications.
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lee2016sensorsinvestigation Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Taek Lee;Tae-Hyung Kim;Jinho Yoon;Yong-Ho Chung;Ji Young Lee;Jeong-Woo Choi;Lee, Taek;Kim, Tae-Hyung;Yoon, Jinho;Chung, Yong-Ho;Lee, Ji Young;Choi, Jeong-Woo;
Journal sensors
Year 2016
DOI
10.3390/s16050660
URL
https://www.mdpi.com/1424-8220/16/5/660
Keywords
Cyclic voltammetry hemoglobin electrochemical biosensor gold nanoparticle au micro-gap

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