Visualization of MG53-mediated Cell Membrane Repair Using in vivo and in vitro Systems

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ID: 266446
2011
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Abstract
Repair of acute injury to the cell membrane is an elemental process of normal cellular physiology, and defective membrane repair has been linked to many degenerative human diseases. The recent discovery of MG53 as a key component of the membrane resealing machinery allows for a better molecular understanding of the basic biology of tissue repair, as well as for potential translational applications in regenerative medicine. Here we detail the experimental protocols for exploring the in vivo function of MG53 in repair of muscle injury using treadmill exercise protocols on mouse models, for testing the ex vivo membrane repair capacity by measuring dye entry into isolated muscle fibers, and for monitoring the dynamic process of MG53-mediated vesicle trafficking and cell membrane repair in cultured cells using live cell confocal microscopy.
Reference Key
ma2011journalvisualization Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Noah Weisleder,Peihui Lin,Xiaoli Zhao,Matthew Orange,Hua Zhu,Jianjie Ma;Noah Weisleder;Peihui Lin;Xiaoli Zhao;Matthew Orange;Hua Zhu;Jianjie Ma;
Journal journal of visualized experiments
Year 2011
DOI
10.3791/2717
URL
https://sciprofiles.com/publication/view/af623b2ff7d8b4cdff6efe6af28805bb
Keywords
cell biology mouse vesicle trafficking confocal microscopy muscle injury issue 52 cell membrane tissue repair treadmill mg53

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