Light autoradiographic localisation of cholinergic muscarinic receptors in rat brain by specific binding of a potent antagonist

Clicks: 178
ID: 266028
1970
Free PDF
Article Quality & Performance Metrics
Overall Quality Improving Quality
0.0 /100
Combines engagement data with AI-assessed academic quality
AI Quality Assessment
Not analyzed
Abstract
THE presence of cholinergic muscarinic receptors on neurones in many regions of the mammalian brain is known from classical electrophysiological studies, which showed that neuronal firing rates are altered by the microiontophoretic application of cholinergic muscarinic agonists and antagonists1. It is now possible to measure these receptors directly by biochemical procedures, some of which involve studying the responses of the cyclic guanosine monophosphate system in neuronal tissue to agonists and antagonists2–5. Other procedures utilised radiolabelled muscarinic agonists and antagonists that bind specifically and directly to presumed receptor sites6–12. One of these agonists13,14, 3-quinuclidinyl-benzilate (QNB) binds in a radiolabelled form to apparent muscarinic receptors in vitro6,7. Recent experiments indicate that it is also possible to demonstrate specific 3H-QNB binding to cholinergic muscarinic receptors in rat brain in vivo soon after intravenous administration15. Because of this latter factor, we have been able to examine the in vivo localisation of 3H-QNB in regions of rat brains by light microscopic autoradiography. We have found receptor sites associated with various groups of cells, some of which are known to be cholinoceptive. In general, the greatest densities of 3H-QNB sites were in telencephalic regions.
Reference Key
kuhar1970naturelight Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors MICHAEL J. KUHAR;HENRY I. YAMAMUR;MICHAEL J. KUHAR;HENRY I. YAMAMUR;
Journal Nature
Year 1970
DOI
doi:10.1038/253560a0
URL
https://doi.org/10.1038/253560a0
Keywords
Science humanities and social sciences multidisciplinary National Center for Biotechnology Information NCBI NLM MEDLINE animals pubmed abstract nih national institutes of health national library of medicine research support u.s. gov't male P.H.S. Rats Receptors hippocampus / metabolism histocytochemistry brain / metabolism* tritium autoradiography m j kuhar cerebral cortex / metabolism corpus striatum / metabolism brain / enzymology hypothalamus / metabolism cholinergic* mesencephalon / metabolism pmid:1117989 doi:10.1038/253560a0 h i yamamura acetylcholinesterase / analysis acetyltransferases / analysis benzilates / metabolism choline parasympatholytics / metabolism parasympathomimetics / metabolism quinuclidines / metabolism* thalamus / metabolism

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.