Structural Stabilities and Transformation Mechanism of Rhynchophylline and Isorhynchophylline by Ultra Performance Liquid Chromatography/Time-of-Flight Mass Spectrometry (UPLC/Q-TOF-MS)

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ID: 265896
2015
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Abstract
To reveal the structural stabilities and transformation mechanism of rhynchophylline (RIN) and isorhynchophylline (IRN), HPLC and UPLC-Q-TOF-MS method were developed for the qualitative and quantitative analysis of the conversion rate. The method was validated for linearity, inter- and intra-day precisions, repeatability and stability. All the quantitative determination method validation results were satisfactory. Under the optimized chromatographic conditions, the effect of various heat temperatures, retention time, and solvent polarities on conversion rate and equilibrium were systematically investigated for the first time. Besides, a model relating the retention yield value and time-temperature was built to predict the t0.5 and Ea of the conversion rate by the Arrhenius equation. The experimental results proved to be in good accordance with the predicted values. Furthermore, UPLC-Q-TOF-MS analysis was performed to verify the transformation mechanism and provide valuable information for stability analysis of the conversion products.
Reference Key
wu2015moleculesstructural Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhen-Feng Wu;Ya-Qi Wang;Na Wan;Gang Ke;Peng-Fei Yue;Hao Chen;Juan-Juan Zhan;Ming Yang;Wu, Zhen-Feng;Wang, Ya-Qi;Wan, Na;Ke, Gang;Yue, Peng-Fei;Chen, Hao;Zhan, Juan-Juan;Yang, Ming;
Journal molecules
Year 2015
DOI
10.3390/molecules200814849
URL
https://doi.org/10.3390/molecules200814849
Keywords
stability uplc-q-tof-ms conversion rate rhynchophylline isorhynchophylline stability uplc-q-tof-ms conversion rate rhynchophylline isorhynchophylline

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