[Identification of the side population associated with ATP-binding cassette transporters activity using imaging flow cytometry].

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ID: 265452
2021
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Abstract
DyeCycle Violet efflux, caused by ATP-binding cassette transporters activity, was analyzed in human colorectal adenocarcinoma cell lines SW480, HT-29, Caco-2 by neans of FACSAria III flow cytometer and ImageStreamX Mk II imaging flow cytometer. Along with similarity of cytometry data obtained on the two instruments, the use of imaging flow cytometry made it possible to characterize the morphology of side population cells, as well as morphology of other cell populations differing in the degree of dye accumulation. The population of cells, which are smaller than the side population cells and practically do not take the dye, is of the special interest. Probably, this population may contribute to the tumor resistance to chemotherapy.
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gisina2021identificationbiomeditsinskaia Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Gisina, A M;Kim, Y S;Yarygin, K N;Lupatov, A Yu;
Journal Biomeditsinskaia khimiia
Year 2021
DOI
10.18097/PBMC20216702137
URL
Keywords
side population dyecycle violet colorectal adenocarcinoma imaging flow cytometry

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