Production and purification of Clostridium perfringens type D epsilon toxin and IgY antitoxin.

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ID: 265127
2021
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Abstract
The aim of this study was to purify Clostridium perfringens type D epsilon toxin and produce and purify anti-epsilon chicken immunoglobulin Y (IgY). A single-step ion exchange chromatography resulted in a high-yield and high-purity toxin, while ion exchange chromatography followed by gel filtration resulted in the highest purity of the toxin, but at a lower yield. Purified and inactivated epsilon toxin were then administered in chickens via four inoculations and IgY was obtained at a high purity and yield, with an antibody titer of 50 IU/mL and high levels of avidity (73.2%). In summary, C. perfringens type D epsilon toxin and chicken anti-epsilon IgY were successfully produced and purified, and may be used for the diagnosis of enterotoxemia caused by the epsilon toxin, as well as in potency tests of existing and future vaccines against enterotoxemia.
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Authors Alves, Guilherme Guerra;Gonçalves, Luciana Aramuni;Assis, Ronnie Antunes;Oliveira Júnior, Carlos Augusto de;Silva, Rodrigo Otávio Silveira;Heneine, Luiz Guilherme Dias;Lobato, Francisco Carlos Faria;
Journal Anaerobe
Year 2021
DOI
S1075-9964(21)00037-8
URL
Keywords
vaccine antibody chromatography enterotoxemia igy

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