In vitro potency test for evaluation of Clostridium perfringens type D epsilon toxoid
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Abstract
The control of enterotoxemia caused by the epsilon toxin, produced by Clostridium perfringenstype D, is based on vaccination with epsilon toxoid. The potency test for this immunogen is conducted using seroneutralization in mice. Here, an in vitro test for detection of neutralizing antibodies with Madin-Darby Canine Kidney (MDCK) cells was standardized in order to study alternative methodologies for the potency test. Titers observed in the in vivo and in vitro seroneutralization tests had a correlation of 99.73%.
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| Authors | Salvarini, Felipe Masiero;Lobato, Zélia Ines Portela;Pires, Prhiscylla Sadanã;Silva, Rodrigo Otávio Silveira;Alves, Guilherme Guerra;Pereira, Pedro Lúcio Lithg;Lobato, Francisco Carlos Faria; |
| Journal | arquivos do instituto biológico |
| Year | 0000 |
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