Plant protein hydrolysates as fish fry feed in aquaculture. Hydrolysis of rapeseed proteins by an enzyme complex from king crab hepatopancreas

Clicks: 225
ID: 262672
2017
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Abstract
Rapeseed proteins were processed by an enzyme complex isolated from king crab hepatopancreas in order to obtain a hydrolysate for use as fish fry feed. The amino acid composition of the obtained protein preparation was close to the amino acid composition of fishmeal traditionally used in the production of fish feed. SDS-PAGE, HPLC, and mass spectrometric analysis of the products of enzymatic hydrolysis of rapeseed proteins showed that the proteins were hydrolyzed to a high degree. The composition of the hydrolysates depended on the hydrolysis time and included free amino acids (27% of the total weight of the protein mix after 3 h of hydrolysis and 56% after 21 h of hydrolysis), short peptides (2 to 20 amino acid residues), and small amounts of protein fragments with a molecular weight of approximately 14 kDa, as shown by by SDS-PAG electrophoresis.
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muranova2017appliedplant Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors T. A. Muranova;D. V. Zinchenko;S. V. Kononova;N. A. Belova;A. I. Miroshnikov;T. A. Muranova;D. V. Zinchenko;S. V. Kononova;N. A. Belova;A. I. Miroshnikov;
Journal applied biochemistry and microbiology
Year 2017
DOI
doi:10.1134/S0003683817060102
URL
https://link.springer.com/article/10.1134/S0003683817060102
Keywords
Microbiology biochemistry medical microbiology general

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