Biosensor for the detection of bacteriophages based on a super-high-frequency resonator

Clicks: 223
ID: 261628
2017
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Emerging

Ranked #17 of 28 articles by views in applied biochemistry and microbiology

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Azospirillum lipoferum Sp59b microbial cells were immobilized on the surface of thin polystyrene films modified in plasma from a high-frequency discharge of argon (13.56 MHz). The optimal conditions for immobilization, under which cell activity was maintained with respect to specific bacteriophages, were established. It was shown that it is possible to record the interaction of immobilized microbial cells and bacteriophages with a microwave-based resonant system (5–8.5 GHz). It was found that the biosensor made it possible to distinguish the interaction of bacterial cells with specific bacteriophages from the control, in which such interaction was absent. With the obtained super-high-frequency sensor, it was possible to determine the content of ΦAl-Sp59b bacteriophages in a suspension containing ~106 phages/mL. The analysis time was about 10 min. The viability of microbial cells after immobilization was also determined with this sensor. The results obtained with the use of the sensor on the basis of a super-high-frequency resonator have shown that it is promising for the development of methods to determine viral particles and the viability of microbial cells.
Reference Key
guliy2017appliedbiosensor Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors O. I. Guliy;B. D. Zaitsev;A. V. Smirnov;O. A. Karavaeva;I. A. Borodina;O. I. Guliy;B. D. Zaitsev;A. V. Smirnov;O. A. Karavaeva;I. A. Borodina;
Journal applied biochemistry and microbiology
Year 2017
DOI
doi:10.1134/S0003683817060084
URL
https://link.springer.com/article/10.1134/S0003683817060084
Keywords
Microbiology biochemistry medical microbiology general

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.