Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity

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ID: 261538
2017
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Abstract
Defensins are abundant in cells and tissues that are involved in host defense against microbial infections. zfDB3 (zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish (Danio rerio). Here we focus on mzfDB3, which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized mzfDB3 gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into Pichia pastoris X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide (rmzfDB3) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product (rmzfDB3, 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed antibacterial activity against Grampositive (i.e., Listeria monocytogenes, Staphylococcus aureus, and Bacillus cereus) and Gram-negative (i.e., Escherchia coli BL21, Vibrio parahaemolyticus, Salmonella lignieres, and Pseudomonas aeruginosa) bacteria.
Reference Key
tao2017appliedexpression Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Y. Tao;C. F. Song;W. Li;Y. Tao;C. F. Song;W. Li;
Journal applied biochemistry and microbiology
Year 2017
DOI
doi:10.1134/S0003683817060126
URL
https://link.springer.com/article/10.1134/S0003683817060126
Keywords
Microbiology biochemistry medical microbiology general

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