Synthesis and "DNA Interlocks" Formation of Small Circular Oligodeoxynucleotides.

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ID: 261315
2020
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Abstract
Circular oligodeoxynucleotides (c-ODNs) have their particular characteristics in topological properties. However, different from oligoribonucleotides, enzymatic synthesis of small c-ODNs is still challenging using conventional methods. Herein, we successfully achieved highly efficient cyclization of linear single-stranded ODNs using T4 DNA ligase simply through the frozen/lyophilization/cyclization (FLC) method. We successfully shortened the cyclization length of linear ODNs to 20 nt (l-ODN 20) with up to 63% yield, which was never achieved before through normal enzymatic methods. With the efficient FLC method, we further developed "DNA interlocks" which were intercross-linked with multiple c-ODNs using the one-pot FLC method. This FLC strategy provides a powerful, cheap, and convenient method to synthesize small c-ODNs for studying DNA nanotechnology and paves the way to achieve future deciphering of c-ODN functions.
Reference Key
gubu2020synthesisacs Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Gubu, Amu;Wang, Jing;Jin, Hongwei;Tang, Xinjing;
Journal ACS applied materials & interfaces
Year 2020
DOI
10.1021/acsami.0c00923
URL
Keywords
dna nanotechnology dna cyclization dna interlocks flc method circular oligodeoxynucleotide crosslinked dna small c-odn

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