Creation of thermostable polypeptide cassettes for amino acid balancing in farm animal rations

Clicks: 93
ID: 260816
2017
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Abstract
The study of the poultry needs in basic nutrients allowed the development of a scheme for obtaining feed polypeptides (“polypeptide cassettes”) enriched with L-amino acids, which are necessary for the metabolism of birds. The amino acid and nucleotide profiles of about 500 bioinformation sequences of thermostable plant proteins and archaea were studied, on the basis of which candidate sequences were selected. In silico, the amino acid and domain composition of the thermostable polypeptides has been optimized. A library of genetically engineered constructs encoding optimized polypeptides with the necessary composition of L-amino acids irreplaceable for poultry has been created. Primary E. coli producer strains were obtained, and the expression and thermostability of the target polypeptides were studied.
Reference Key
grishin2017appliedcreation Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors D. V. Grishin;Yu. A. Gladilina;S. S. Aleksandrova;M. V. Pokrovskaya;O. V. Podobed;V. S. Pokrovskii;D. D. Zhdanov;N. N. Sokolov;D. V. Grishin;Yu. A. Gladilina;S. S. Aleksandrova;M. V. Pokrovskaya;O. V. Podobed;V. S. Pokrovskii;D. D. Zhdanov;N. N. Sokolov;
Journal applied biochemistry and microbiology
Year 2017
DOI
doi:10.1134/S0003683817060072
URL
https://link.springer.com/article/10.1134/S0003683817060072
Keywords
Microbiology biochemistry medical microbiology general

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