Following Glucose Oxidase Activity by Chemiluminescence and Chemiluminescence Resonance Energy Transfer (CRET) Processes Involving Enzyme-DNAzyme Conjugates

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ID: 260766
2011
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Abstract
A hybrid consisting of glucose oxidase-functionalized with hemin/G-quadruplex units is used for the chemiluminescence detection of glucose. The glucose oxidase-mediated oxidation of glucose yields gluconic acid and H2O2. The latter in the presence of luminol acts as substrate for the hemin/G-quadruplex-catalyzed generation of chemiluminescence. The glucose oxidase/hemin G-quadruplex hybrid was immobilized on CdSe/ZnS quantum dots (QDs). The light generated by the hybrid, in the presence of glucose, activated a chemiluminescence resonance energy transfer process to the QDs, resulting in the luminescence of the QDs. The intensities of the luminescence of the QDs at different concentrations of glucose provided an optical means to detect glucose.
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niazov2011sensorsfollowing Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Angelica Niazov;Ronit Freeman;Julia Girsh;Itamar Willner;Niazov, Angelica;Freeman, Ronit;Girsh, Julia;Willner, Itamar;
Journal sensors
Year 2011
DOI
10.3390/s111110388
URL
https://dx.doi.org/10.3390/s111110388
Keywords
hydrogen peroxide quantum dots glucose sensor chemiluminescence g-quadruplex dnazyme chemiluminescence resonance energy transfer

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