improvement of trehalose production by immobilized trehalose synthase from thermus thermophilus hb27
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2018
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Abstract
Trehalose is a non-reducing disaccharide with a wide range of applications in the fields of food, cosmetics, and pharmaceuticals. In this study, trehalose synthase derived from Thermus thermophilus HB27 (TtTreS) was immobilized on silicalite-1-based material for trehalose production. The activity and the stability of TtTreS against pH and temperature were significantly improved by immobilization. Enzyme immobilization also led to a lower concentration of byproduct glucose, which reduces byproduct inhibition of TtTreS. The immobilized TtTreS still retained 81% of its initial trehalose yield after 22 cycles of enzymatic reactions. The immobilized TtTreS exhibited high operational stability and remarkable reusability, indicating that it is promising for industrial applications.
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sun2018moleculesimprovement
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| Authors | ;Jing Sun;Shizeng Wang;Wenna Li;Ruimin Li;Sheng Chen;Hyon Il Ri;Tae Mun Kim;Myong Su Kang;Lu Sun;Xinxiao Sun;Qipeng Yuan |
| Journal | Journal of ethnopharmacology |
| Year | 2018 |
| DOI |
10.3390/molecules23051087
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