culture conditions influence satellite cell activation and survival of single myofibers

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2018
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Abstract
Single myofiber isolation protocols allow to obtain an in vitro system in which the physical association between the myofiber and its stem cells, the satellite cells, is adequately preserved. This technique is an indispensable tool by which the muscle regeneration process can be recapitulated and studied in each specific phase, from satellite cell activation to proliferation, from differentiation to fusion. This study aims to clarify the effect of different culture conditions on single myofibers, their associated satellite cells, and the physiological behavior of the satellite cells upon long term culture. By direct observations of the cultures, we compared different experimental conditions and their effect on both satellite cell behavior and myofiber viability.
Reference Key
renzini2018europeanculture Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Alessandra Renzini;Anna Benedetti;Marina Bouché;Leopoldo Silvestroni;Sergio Adamo;Viviana Moresi
Journal journal of applied mathematics
Year 2018
DOI
10.4081/ejtm.2018.7567
URL
https://pagepressjournals.org/index.php/bam/article/view/7567
Keywords
proliferation growth factors cell adhesion

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