influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires influência da temperatura, tempo de incubação, densidade do antígeno e idade da cultura na aglutinabilidade das leptospiras

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ID: 257354
2003
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Abstract
In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.
Na leptospirose, o teste de soroaglutinação microscópica (MAT) é a base do diagnóstico sorológico, sendo amplamente usado. Em nosso estudo, o MAT foi aplicado para avaliar a influência da temperatura, tempo de incubação, densidade do antígeno, a idade da cultura na aglutinabilidade das leptospiras e a interferência desses fatores na interpretação do diagnóstico. Três amostras de soro com títulos de 100, 800 e 25600 para o sorovar tande (isolado local), do sorogrupo canicola foram utilizadas com combinações diferentes entre os quatro fatores. Houve uma significante relação entre todos os fatores principalmente a densidade do antígeno e a idade da cultura.
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Authors ;Núbia Seyffert;Éverton Fagonde da Silva;Viviane Lorenzato Bermudez;Claudiomar Soares Brod
Journal ieee software
Year 2003
DOI
10.1590/S1517-83822003000500025
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500025
Keywords
leptospirosis leptospira laboratorial diagnosismicrobiology

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