A label-free optical whole-cell Escherichia coli biosensor for the detection of pyrethroid insecticide exposure.

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ID: 25684
2019
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Abstract
There is a growing need for low-cost, portable technologies for the detection of threats to the environment and human health. Here we propose a label-free, optical whole-cell Escherichia coli biosensor for the detection of 3-phenoxybenzoic acid (3-PBA), a biomarker for monitoring human exposure to synthetic pyrethroid insecticides. The biosensor functions like a competitive ELISA but uses whole-cells surface displaying an anti-3-PBA VHH as the detection element. When the engineered cells are mixed with 3-PBA-protein conjugate crosslinking that can be visually detected occurs. Free 3-PBA in samples competes with these crosslinks, leading to a detectable change in the output. The assay performance was improved by coloring the cells via expression of the purple-blue amilCP chromoprotein and the VHH expression level was reduced to obtain a limit of detection of 3 ng/mL. The optimized biosensor exhibited robust function in complex sample backgrounds such as synthetic urine and plasma. Furthermore, lyophilization enabled storage of biosensor cells for at least 90 days without loss of functionality. Our whole-cell biosensor is simple and low-cost and therefore has potential to be further developed as a screening tool for monitoring exposure to pyrethroids in low-resource environments.
Reference Key
riangrungroj2019ascientific Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Riangrungroj, Pinpunya;Bever, Candace Spier;Hammock, Bruce D;Polizzi, Karen M;
Journal Scientific reports
Year 2019
DOI
10.1038/s41598-019-48907-6
URL
Keywords Keywords not found

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