Dark-Field Microscopic Detection of Bacteria Using Bacteriophage-Immobilized SiO@AuNP Core-Shell Nanoparticles.

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ID: 25682
2019
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Abstract
To replace molecular biological and immunological methods, biosensors have recently been developed for the rapid and sensitive detection of bacteria. Among a wide variety of biological materials, bacteriophages have received increasing attention as promising alternatives to antibodies in biosensor applications. Thus, we herein present a rapid and highly selective detection method for pathogenic bacteria, which combines dark-field light scattering imaging with a plasmonic biosensor system. The plasmonic biosensor system employs bacteriophages as the biorecognition element and the aggregation-induced light scattering signal of gold nanoparticle-assembled silica nanospheres as a signal transducer. Using strain SA27 as a model analyte, we demonstrated that the plasmonic biosensor system detects in the presence of excess in a highly selective manner. After mixing of the sample and the phage S13'-conjugated plasmon scattering probe, detection was complete within 15-20 min with a detection limit of 8×10 colony forming units per milliliter.
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imai2019darkfieldanalytical Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Imai, Masashi;Mine, Kouhei;Tomonari, Haruna;Uchiyama, Jumpei;Matsuzaki, Shigenobu;Niko, Yosuke;Hadano, Shingo;Watanabe, Shigeru;
Journal Analytical chemistry
Year 2019
DOI
10.1021/acs.analchem.9b02715
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