Dependent signal quenching and enhancing triggered by bipedal DNA walker for ultrasensitive photoelectrochemical biosensor.

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ID: 25681
2019
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Abstract
Herein, by utilizing bipedal DNA walker as booster to adjust the distance of quencher ferrocene (Fc) and sensitizer methylene blue (MB) to photoactive material perylene-3,4,9,10-tetracarboxylic acid (PTCA), a novel "on-off-super on" photoelectrochemical (PEC) biosensor was proposed for ultrasensitive detection of thrombin (TB). Firstly, the PTCA matrix on electrode could provide a high initial PEC signal assisted by depAu. Upon the Fc labeled on hairpin DNA 1 (H-Fc) proximate to PTCA, the PEC signal could obviously decrease to reduce the background signal. Interestingly, the target TB-related bipedal DNA walker implemented the opening of H-Fc for the departure of Fc toward PTCA, which achieved the recovery of PEC signal and exposed the prelocked toehold domain for the hybridization with hairpin DNA 2 labeled with MB (H-MB), thereby making the MB approach to PTCA for achieving the "super on" signal. As a result, this proposed strategy showed a wide linear range from 0.5 fM to 100 nM with a low detection limit down to 0.17 fM for TB detection, providing an efficient and available avenue for sensitive detection of biomolecules in bioanalysis and disease diagnosis.
Reference Key
zhu2019dependentbiosensors Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Zhu, Minghui;Zhong, Xia;Deng, Hanmei;Huang, Liaojing;Yuan, Ruo;Yuan, Yali;
Journal Biosensors & bioelectronics
Year 2019
DOI
S0956-5663(19)30697-9
URL
Keywords Keywords not found

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