fabrication of alkoxyamine-functionalized magnetic core-shell microspheres via reflux precipitation polymerization for glycopeptide enrichment
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ID: 256424
2016
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Abstract
As a facile method to prepare hydrophilic polymeric microspheres, reflux precipitation polymerization has been widely used for preparation of polymer nanogels. In this article, we synthesized a phthalamide-protected N-aminooxy methyl acrylamide (NAMAm-p) for preparation of alkoxyamine-functionalized polymer composite microspheres via reflux precipitation polymerization. The particle size and functional group density of the composite microspheres could be adjusted by copolymerization with the second monomers, N-isopropyl acrylamide, acrylic acid or 2-hydroxyethyl methacrylate. The resultant microspheres have been characterized by TEM, FT-IR, TGA and DLS. The experimental results showed that the alkoxyamine group density of the microspheres could reach as high as 1.49 mmol/g, and these groups showed a great reactivity with ketone/aldehyde compounds. With the aid of magnetic core, the hybrid microspheres could capture and magnetically isolate glycopeptides from the digested mixture of glycopeptides and non-glycopeptides at a 1:100 molar ratio. After that, we applied the composite microspheres to profile the glycol-proteome of a normal human serum sample, 95 unique glycopeptides and 64 glycoproteins were identified with these enrichment substrates in a 5 μL of serum sample.
| Reference Key |
yu2016polymersfabrication
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|---|---|
| Authors | ;Meng Yu;Yi Di;Ying Zhang;Yuting Zhang;Jia Guo;Haojie Lu;Changchun Wang |
| Journal | Journal of Fluorescence |
| Year | 2016 |
| DOI |
10.3390/polym8030074
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| URL | |
| Keywords | Keywords not found |
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