microsatellite analysis of cryopreserved stallion semen stored on fta(r) paper : research communication
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ID: 256242
2002
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Abstract
The aim of this study was to establish and validate a method to permit microsatellite analysis of DNA profiles obtained from frozen-thawed stallion sperm cells. This would provide reliable and accurate verification of the identification of a semen donor. Ejaculates from 5 pony stallions were collected, processed and frozen in 0.5 m plastic straws. Aliquots of 100 m of the frozen-thawed semen thus obtained were either placed directly, or diluted (1 : 10 ; 1 : 100 ; and 1 : 1000) and placed on slides of FTA(R) paper. Similarly, blood samples obtained from each of the stallions were placed onto slides of FTA(R) paper. A punch was removed from each sample after drying. Each sample was mixed with FTA(R) purification reagent, Dithiothreitol and Proteinase K before incubation and processing. All samples were processed with a set of 13 microsatellite markers. Further analysis permitted a comparison of the DNA profiles of the frozen-thawed semen and the blood samples. A full profile of markers was obtained from the 1 : 10 and 1 : 100 dilutions of the frozen-thawed semen samples as well as from the blood samples. The DNA profiles from the frozen-thawed semen and blood samples obtained from the stallions matched in all cases.
| Reference Key |
schulman2002journalmicrosatellite
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|---|---|
| Authors | ;M.L. Schulman;C.K. Harper;E. Bell;A. Nel;A.J. Guthrie |
| Journal | race: revista de administração, contabilidade e economia |
| Year | 2002 |
| DOI |
10.4102/jsava.v73i4.592
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