matrix evaluation for pseudomonas spp. immobilisation in phenol bioremediation
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2003
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Abstract
Pseudomonas spp. were cultivated in a free cell suspension and also immobilised in three different matrices to observe the influence of a contaminant like phenol on degradation velocity and compare each one's results. Polyurethane polymers, alginate (Manohar et al, 2001) and a mixture of alginate and polyvinyl alcohol (Doria et al, 2002) were selected and tested as matrices; all of them proved viable as matrices for cell immobilisation. Pseudomonas were cultivated in an initial 10 cfu/ml concentration in each one of the matrices for comparison purposes and in a medium without matrix; all mediums were supplemented with a minimum salt medium and 200 ppm phenol. A removal time of 23 days was observed in the medium without matrix, 15 days in the polyurethane matrix and 7 days in the alginate matrices. Improved removal times were observed in all matrices when compared to the free cell suspension.
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| Authors | ;Leonel Chitiva Urbina;Jenny Dussán |
| Journal | gigascience |
| Year | 2003 |
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