estudo comparativo entre as tÉcnicas de schalm e de clauss na determinaÇÃo da concentraÇÃo plasmÁtica do fibrinogÊnio em eqÜinos hÍgidos e com cÓlica comparative study between plasma fibrinogen concentrations determined by schalm and clauss techniques in colic and healthy horses

Clicks: 164
ID: 255352
2009
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Abstract
<div>Foram comparadas, em oito diferentes momentos, duas técnicas de determinação da concentração plasmática do fibrinogênio em equinos hígidos (GI=20) e com cólica (GII=10): a técnica de Schalm, fundamentada na precipitação térmica do fibrinogênio a 56oC, e a técnica de Clauss, que envolve a polimerização da molécula de fibrinogênio. Em ambos os grupos e em todos os momentos de avaliação os valores médios obtidos por intermédio da técnica de Schalm foram superiores (P<0,05) àqueles obtidos pela técnica de Clauss. A diferença observada entre os dois métodos avaliados deve-se à interferência de inúmeros fatores inibidores do processo cinético atuantes no procedimento de Clauss. A hiperfibrinogenemia apresentada pelos animais com cólica (GII) entre o segundo e o quinto dias pós-operatório deve-se não somente ao trauma cirúrgico, mas também ao processo inflamatório e infeccioso da ferida cirúrgica. A análise seriada do fibrinogênio contribui de forma positiva e precoce no diagnóstico de processos inflamatórios e infecciosos em equinos com cólica. Diante de tais observações, recomenda-se o método de Schalm na determinação do fibrinogênio em equinos hígidos e com cólica, por ser de um procedimento simples, de fácil execução, baixo custo e com resultados coerentes.</div><div><br /></div><div>PALAVRAS-CHAVES: Cólica, comparação de técnicas, equinos, fibrinogênio.</div><div><br /></div> <div>Two different techniques for the determination of fibrinogen plasma concentration were compared during eight time points. Twenty healthy horses (GI) and ten horses with colic (GII) were included in this study. The Schalm´s technique was compared to the Clauss´s technique. The first one is based on the thermal precipitation of the fibrinogen at 56oC. The second involves the polymerization of the fibrinogen molecule. Both groups of horses during all time points had mean values of plasmatic fibrinogen higher using Schalm’s technique compared to the values obtained with Clauss technique (p<0.05). This difference can be due to the several inhibitor factors that interfere with the kinetic process at the Clauss´s technique. The hyperfibrinogenemia seen in GII, between the 2nd and 5th day post-surgery occurred not only due to the surgical trauma, but also to the infection process at the surgical wound. With these finding it is recommended the use of Schalm’s technique for fibrinogen determination in healthy and colic horses. This technique is then considered to be the simplest, of easy execution, low cost and coherent. The seriated analysis of fibrinogen contributes in a positive and precocious manner during the diagnoses of inflammatory and infectious processes in colic horses. </div><div><br /></div><div>KEY WORD: Colic, equine, fibrinogen, techniques comparison.</div><div><br /></div>
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santana2009cinciaestudo Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Aureo Evangelista Santana;Paula Alessandra Di Filippo;Lia G. Rezende
Journal proceedings of the winter simulation conference winter simulation conference
Year 2009
DOI
DOI not found
URL
http://www.revistas.ufg.br/index.php/vet/article/view/3265
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