Single-Cell Landscape of Transcriptional Heterogeneity and Cell Fate Decisions during Mouse Early Gastrulation

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ID: 25449
2017
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Abstract
Summary: The mouse inner cell mass (ICM) segregates into the epiblast and primitive endoderm (PrE) lineages coincident with implantation of the embryo. The epiblast subsequently undergoes considerable expansion of cell numbers prior to gastrulation. To investigate underlying regulatory principles, we performed systematic single-cell RNA sequencing (seq) of conceptuses from E3.5 to E6.5. The epiblast shows reactivation and subsequent inactivation of the X chromosome, with Zfp57 expression associated with reactivation and inactivation together with other candidate regulators. At E6.5, the transition from epiblast to primitive streak is linked with decreased expression of polycomb subunits, suggesting a key regulatory role. Notably, our analyses suggest elevated transcriptional noise at E3.5 and within the non-committed epiblast at E6.5, coinciding with exit from pluripotency. By contrast, E6.5 primitive streak cells became highly synchronized and exhibit a shortened G1 cell-cycle phase, consistent with accelerated proliferation. Our study systematically charts transcriptional noise and uncovers molecular processes associated with early lineage decisions. : Mohammed et al. chart mouse embryonic development from implantation to early gastrulation at single-cell resolution. They describe regulatory processes associated with lineage commitment. An increased level of transcriptional noise is observed prior to lineage commitment, an observation that provides fresh insights into cell fate decision-making processes. Keywords: gastrulation, embryo, single-cell RNA-seq, epiblast, primitive endoderm, primitive streak, X-chromosome, transcriptional noise
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mohammed2017singlecellcell Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Mohammed, Hisham;Hernando-Herraez, Irene;Savino, Aurora;Scialdone, Antonio;Macaulay, Iain;Mulas, Carla;Chandra, Tamir;Voet, Thierry;Dean, Wendy;Nichols, Jennifer;Marioni, John C.;Reik, Wolf;
Journal Cell reports
Year 2017
DOI
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URL
http://www.sciencedirect.com/science/article/pii/S2211124717309610
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