a tet-on and cre-loxp based genetic engineering system for convenient recycling of selection markers in penicillium oxalicum

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ID: 253868
2016
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Ranked #356 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Abstract
The lack of selective markers has been a key problem preventing multistep genetic engineering in filamentous fungi, particularly for industrial species such as the lignocellulose degrading Penicillium oxalicum JUA10-1(formerly named as Penicillium decumbens). To resolve this problem, we constructed a genetic manipulation system taking advantage of two established genetic systems: the Cre-loxP system and Tet-on system in Penicillium oxalicum JUA10-1. This system is simple and efficient. The expression of Cre recombinase was activated by doxycycline since it was controlled by Tet-on system. Using this system, two genes, ligD and bglI, were sequentially disrupted by loxP flanked ptrA. The successful application of this procedure will provide a useful tool for genetic engineering in filamentous fungi. This system will also play an important role in improving the productivity of interesting products and minimizing by-product when fermented by filamentous fungi.
Reference Key
ejiang2016frontiersa Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Baojie eJiang;Ruiqin eZhang;Dan eFeng;Fangzhong eWang;Kuimei eLiu;Yi eJiang;Kangle eNiu;Quanquan eYuan;Mingyu eWang;Hailong eWang;Hailong eWang;Youming eZhang;Youming eZhang;Xu eFang
Journal journal of magnetic resonance (san diego, calif : 1997)
Year 2016
DOI
10.3389/fmicb.2016.00485
URL
http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00485/full
Keywords
doxycycline selective markersmicrobiology

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