investigating the effect of growth phase on the surface-layer associated proteome of lactobacillus acidophilus using quantitative proteomics

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2017
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Ranked #462 of 875 articles by views in journal of magnetic resonance (san diego, calif : 1997)

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Abstract
Bacterial surface-layers (S-layers) are semi-porous crystalline arrays that self-assemble to form the outermost layer of some cell envelopes. S-layers have been shown to act as scaffolding structures for the display of auxiliary proteins externally. These S-layer associated proteins have recently gained attention in probiotics due to their direct physical contact with the intestinal mucosa and potential role in cell proliferation, adhesion, and immunomodulation. A number of studies have attempted to catalog the S-layer associated proteome of Lactobacillus acidophilus NCFM under a single condition. However, due to the versatility of the cell surface, we chose to employ a multiplexing-based approach with the intention of accurately contrasting multiple conditions. In this study, a previously described lithium chloride isolation protocol was used to release proteins bound to the L. acidophilus S-layer during logarithmic and early stationary growth phases. Protein quantification values were obtained via TMT (tandem mass tag) labeling combined with a triple-stage mass spectrometry (MS3) method. Results showed significant growth stage-dependent alterations to the surface-associated proteome while simultaneously highlighting the sensitivity and reproducibility of the technology. Thus, this study establishes a framework for quantifying condition-dependent changes to cell surface proteins that can easily be applied to other S-layer forming bacteria.
Reference Key
klotz2017frontiersinvestigating Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors Courtney Klotz, Sarah O'Flaherty, Yong Jun Goh, Rodolphe Barrangou
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Journal journal of magnetic resonance (san diego, calif : 1997)
Year 2017
DOI
10.3389/fmicb.2017.02174
URL
http://journal.frontiersin.org/article/10.3389/fmicb.2017.02174/full
Keywords
probiotic lactobacillus

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