screening jatropha genotypes for aluminum tolerance using the solution-paper method
seleção de genótipos de pinhão-manso tolerantes ao alumínio usando o método papel-solução

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ID: 252212
2012
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Abstract
The identification of genetic material tolerant to aluminum (Al) can offer important advantages to breeding programs and to recommendations for cultivars with the aim of utilizing acidic soil. Based on this, our aim was to determine, by using the solution-paper method, the aluminum concentration that best demonstrates tolerance of Jatropha accessions to this element. For this study, seeds from eight Jatropha accessions (CNPAE 114, CNPAE 154, CNPAE 169, CNPAE 178, CNPAE 218, CNPAE 200, CNPAE 226, CNPAE 228) were placed in a germination chamber with different concentrations of Al (0, 85, 155, 225 and 295 mg L-1) using the solution-paper technique. After 14 days, the length of the primary root was evaluated and the percentage by which those subjected to doses of Al was calculated. A concentration of 85 mg L-1 was the level that separated the accessions in terms of Al tolerance; the accessions CNPAE 114 and CNPAE 154 were the most sensitive to the presence of this toxic element in the solution. It was concluded that the solution-paper method is efficient and can be used in Jatropha breeding programs for the selection of Al-tolerant genotypes.

A identificação de material genético tolerante ao alumínio (Al) pode oferecer importantes subsídios aos programas de melhoramento e à recomendação de cultivares destinada a utilização de solos ácidos. Assim, o objetivo deste trabalho foi determinar, através do método de papel-solução, a concentração de alumínio que melhor discrimine acessos de pinhão-manso quanto à tolerância ao Al. Para este estudo, sementes de oito acessos de pinhão-manso (CNPAE 114, CNPAE 154, CNPAE 169, CNPAE 178, CNPAE 218, CNPAE 200, CNPAE 226, CNPAE 228) foram colocadas para germinar em diferentes concentrações de Al (0, 85, 155, 225 e 295 mg L-1) utilizando a técnica do papel solução. Após 14 dias o comprimento da raiz primária foi avaliado e a porcentagem de redução foi calculada para os sujeitados as doses de Al. A concentração de 85 mg L-1 foi a que melhor separou os acessos, em termos de tolerância ao Al, e que os acessos CNPAE 114 e CNPAE 154 foram os mais sensíveis à presença do elemento tóxico na solução. Concluiu-se que o método do papel solução é eficiente e pode ser usado em programa de melhoramento de pinhão-manso visando a seleção de genótipos tolerantes ao alumínio.

Reference Key
rosado2012semina:screening Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Tatiana Barbosa Rosado;Bruna Anair Souto Dias;Gustavo Sessa Fialho;Renato Domiciano Silva Rosado;Herminia Emilia Prieto Martinez;Bruno Galvêas Laviola
Journal frontiers in neuroendocrinology
Year 2012
DOI
10.5433/1679-0359.2012v33n4p1273
URL
http://www.uel.br/revistas/uel/index.php/semagrarias/article/view/6828
Keywords
Agriculture (General) jatropha curcas melhoramento toxidade

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