studies regarding the crioprotective proprieties of the vitrification media, with ethylene glycol, sucrose, ficoll 70 and galactose used in mammalian embryo cryopreservation
Clicks: 338
ID: 251051
2009
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This
article has not been analysed, so there is no overall score —
reader engagement is measured and shown alongside.
Reader Engagement
Steady Performance
30.0
/100
338 views
52 readers
AI Quality Assessment
Not analyzed
Readership in this journal
SteadyRanked #14 of 249 articles by views in Enzyme and microbial technology
Most read
Least read
Bar heights use a square-root scale. Only the 120 most-read articles are drawn; the journal has 249 in total.
Mint this article as an NFT
Not yet mintedCreate a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.
5
SUSD
one-off · no wallet required
Abstract
Crioprotectors are the main component of any vitrification media. The penetrant crioprotectors areessential for cell dehydration and for the decrease of the freezing point of the solution, allowing a longertime for dehydration to set in. The aim of our paper was to make a series of experiments in order todetermine the concentration at which four cryoprotectants (ethylene glycol, sucrose, Ficoll 70 andgalactose) singly and in pairs would vitrify on plunging into liquid nitrogen and remain vitreous whenthawed in water bath. A total of 156 solutions were tested. During freezing, vitrification was evidenced bythe formation of transparent glass when the unsealed straws were plunged into liquid nitrogen, at -196°C. Crystallization (ice formation) resulted in a milky appearance. Solutions that vitrify on freezingwere tested if they remain vitreous on thawing. For thawing we tested three temperatures 20°C, 25°C and37°C. During thawing, solutions that did not devitrified were transformed from solid clear state to theliquid state without evidence of a milky appearance. From the combinations of two cryoprotectors weretested a number of 51 solutions vitrify on freezing (19 solutions with ethylene glycol and galactose; 19solutions with ethylene glycol and sucrose; 13 solutions with ethylene glycol and Ficoll). The ethyleneglycol and galacose pair give the best results on thawing (3 combinations remained vitreous on thawing)at 37°C.
| Reference Key |
boleman2009scientificstudies
Use this key to autocite in the manuscript while using
SciMatic Manuscript Manager or Thesis Manager
|
|---|---|
| Authors | ;ALEXANDRA BOLEMAN;N. PĂCALĂ;ADA CEAN |
| Journal | Enzyme and microbial technology |
| Year | 2009 |
| DOI |
DOI not found
|
| URL | |
| Keywords |
Citations
No citations found. To add a citation, contact the admin at info@scimatic.org
Comments
No comments yet. Be the first to comment on this article.