soybean hairy roots produced in vitro by agrobacterium rhizogenes-mediated transformation
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ID: 250033
2018
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Abstract
Soybean is one of the world's most important oil and protein crops. Efficient transformation is a key factor for the improvement of soybean by genetic modification. We describe an optimized protocol for the Agrobacterium rhizogenes-mediated transformation of soybean and the induction of hairy root development in vitro. Cotyledons with 0.5-cm hypocotyls were cut from 5-day-old seedlings and used as explants. After infection and co-cultivation, hairy roots were produced in induction culture medium after 10–12 days. Using this method, 90%–99% of the infected explants of five different cultivars produced hairy roots within one month. Observations using reporter constructs showed that 30%–60% of the hairy roots induced were transformed. Based on high transformation efficiency and short transformation period, this method represents an efficient and rapid platform for study of soybean gene function.
| Reference Key |
chen2018cropsoybean
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|---|---|
| Authors | ;Li Chen;Yupeng Cai;Xiujie Liu;Chen Guo;Shi Sun;Cunxiang Wu;Bingjun Jiang;Tianfu Han;Wensheng Hou |
| Journal | Biomaterials science |
| Year | 2018 |
| DOI |
10.1016/j.cj.2017.08.006
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