entamoeba histolytica: antigenic characterization of axenic strains from brazil entamoeba histolytica: caracterização antigênica de cepas axênicas do brasil

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ID: 248979
1991
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Abstract
Trophozoites from cultures of Entamoeba histolytica strains isolated and grown axenically in Brazil (ICB-CSP, ICB-462 and ICB-32) were used for immune sera production and for characterization of their antigens by using electrophoretic and glycoproteic profiles, in parallel with a standard strain isolated and kept under axenic conditions in USA (HK-9). Hyperimmune sera, presenting high antibody titers with homologous and heterologous antigens, were obtained. The four strains in study revealed similar and complex electrophoretic and glycoproteic profiles showing polypep-tides with molecular weights ranging from 200 to less than 29 kDa. No significant differences were detected between the pathogenic and non-pathogenic strains.
Trofozoítos obtidos de cultura de Entamoeba histolytica isoladas e axenizadas no Brasil (ICB-CSP, ICB-462 e ICB-32) foram utilizados para a produção de soros imunes em coelhos e para a caracterização de antígenos através dos seus perfis eletroforético e glicoproteico, em paralelo com uma cepa padrão isolada e axenizada nos Estados Unidos (HK-9). Obtiveram-se soros hiperimunes, reativos frente aos antígenos homólogos e heterólogos. As quatro cepas em estudo apresentaram perfis eletroforético e glicoproteico complexos e semelhantes, compostos por polipeptídeos com pesos moleculares variando de 200 kDa a menos de 29 kDa. Não se detectaram diferenças significativas entre as cepas patogênicas e não patogênicas.
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Authors Semíramis Guimarães, Haidée Urdaneta, Edward F. Silva, Carlos Alberto P. Tavares
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Journal 2017 12th international conference for internet technology and secured transactions, icitst 2017
Year 1991
DOI
10.1590/S0036-46651991000100002
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651991000100002
Keywords
entamoeba histolytica tropical medicine

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