rna-sequencing analyses demonstrate the involvement of canonical transient receptor potential channels in rat tooth germ development

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ID: 248350
2017
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Abstract
Tooth development depends on multiple molecular interactions between the dental epithelium and mesenchyme, which are derived from ectodermal and ectomesenchymal cells, respectively. We report on a systematic RNA sequencing analysis of transcriptional expression levels from the bud to hard tissue formation stages of rat tooth germ development. We found that GNAO1, ENO1, EFNB1, CALM1, SIAH2, ATP6V0A1, KDELR2, GTPBP1, POLR2C, SORT1, and members of the canonical transient receptor potential (TRPC) channel family are involved in tooth germ development. Furthermore, Cell Counting Kit 8 (CCK8) and Transwell migration assays were performed to explore the effects of these differentially expressed genes (DEGs) on the proliferation and migration of dental pulp stem cells. Immunostaining revealed that TRPC channels are expressed at varying levels during odontogenesis. The identified genes represent novel candidates that are likely to be vital for rat tooth germ development. Together, the results provide a valuable resource to elucidate the gene regulatory mechanisms underlying mammalian tooth germ development.
Reference Key
yang2017frontiersrna-sequencing Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Jun Yang;Wenping Cai;Xi Lu;Shangfeng Liu;Shouliang Zhao
Journal Journal of clinical and experimental dentistry
Year 2017
DOI
10.3389/fphys.2017.00455
URL
http://journal.frontiersin.org/article/10.3389/fphys.2017.00455/full
Keywords
rna-seq tooth development tooth germ

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