cytomegalovirus infection in renal transplant recipients diagnosed by nested-pcr

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ID: 248185
2001
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Abstract
A prospective study of cytomegalovirus (CMV) infection was carried out on 34 renal transplant recipients managed at a General Hospital in Ribeirão Preto, SP, Brazil. Serologic tests showed that all patients were infected with CMV before renal transplantation. Two nested-PCR techniques with primers that recognize sequences of the glycoprotein B (gB) and H (gH) genes were used for CMV detection in blood and urine samples during the post-transplantation period. CMV was detected more frequently in blood samples than in urine samples (P<0.001). Thirty-three patients had CMV detected at least once in blood and/or urine samples. Seven of these patients (21.2%) were diagnosed as having symptomatic CMV infection and showed a worse clinical outcome, with a higher death rate (P = 0.03). No association between CMV viremia and graft rejection was observed. Nested-PCR was not useful to identify patients at risk for symptomatic CMV infection since only 21.2% of the patients with CMV infection were symptomatic.
Reference Key
aquino2001braziliancytomegalovirus Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;V.H. Aquino;L.T.M. Figueiredo
Journal Free radical biology & medicine
Year 2001
DOI
10.1590/S0100-879X2001000100011
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100011
Keywords
polymerase chain reaction cytomegalovirus kidney transplantationmedicine (general)biology (general)

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