multivalent fusion dna vaccine against brucella abortus

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ID: 245275
2017
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Abstract
As an alternative brucellosis prevention method, we evaluated the immunogenicity induced by new multivalent DNA vaccines in BALB/c mice. We constructed the vaccines by fusion of BAB1_0273 and/or BAB1_0278 open reading frames (ORFs) from genomic island 3 (GI-3) and the Brucella abortus 2308 sodC gene with a link based on prolines and alanines (pV273-sod, pV278-sod, and pV273-278-sod, resp.). Results show that immunization with all tested multivalent DNA vaccines induced a specific humoral and cellular immune response. These novel multivalent vaccines significantly increased the production of IgM, IgG, and IgG2a antibodies as well as IFN-γ levels and the lymphoproliferative response of splenocytes. Although immunization with these multivalent vaccines induced a typical T-helper 1- (Th1-) dominated immune response, such immunogenicity conferred low protection levels in mice challenged with the B. abortus 2308 pathogenic strain. Our results demonstrated that the expression of BAB1_0273 and/or BABl_0278 antigens conjugated to SOD protein can polarize mice immunity to a Th1-type phenotype, conferring low levels of protection.
Reference Key
gmez2017biomedmultivalent Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Leonardo Gómez;Javiera Llanos;Emilia Escalona;Darwin Sáez;Francisco Álvarez;Raúl Molina;Manuel Flores;Angel Oñate
Journal spectrochimica acta - part a: molecular and biomolecular spectroscopy
Year 2017
DOI
10.1155/2017/6535479
URL
http://dx.doi.org/10.1155/2017/6535479
Keywords
Medicine

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