different length (dl) qpcr for quantification of cell killing by uv-induced dna damage

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ID: 244468
2010
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Abstract
We describe the different length (DL) qPCR method for quantification of UV induced DNA damage in cell killing. The principle of DL qPCR is that DNA damage inhibits PCR. Applications with different lengths can therefore be used to detect different levels of UV-induced DNA damage. The assay was evaluated on three strains of Escherichia coli exposed to varying levels of ultraviolet (UV) radiation. We show that DL qPCR sensitivity and reproducibility are within the range of practical application to detect the effect of UV cell killing.
Reference Key
skjefstad2010internationaldifferent Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Guro Skjefstad;Bente Carina Johnsrud;Irina Hagen;Knut Rudi;Ingun Tryland
Journal archives of biochemistry and biophysics
Year 2010
DOI
10.3390/ijerph7093376
URL
http://www.mdpi.com/1660-4601/7/9/3376/
Keywords
uv

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