pré-aclimatização in vitro de abacaxi-ornamental in vitro pre-acclimatization of ornamental pineapple

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ID: 243687
2010
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Abstract
O abacaxi ornamental (Ananas bracteatus Schult. f.) é bastante utilizado em composições paisagísticas para delimitar áreas ou canteiros. A micropropagação permite obter altas taxas de multiplicação, podendo ser uma alternativa viável e vantajosa de propagação vegetativa, visto que a demanda por esta espécie vem aumentando, tanto no mercado nacional quanto no internacional. Neste trabalho objetivou-se avaliar a influência de diferentes irradiâncias e concentrações de sacarose durante o enraizamento in vitro, sobre a sobrevivência das mudas na fase de aclimatização. Para tanto, foram testadas diferentes concentrações de sacarose (0, 10, 20 e 30 g L-1) no meio de enraizamento, bem como duas irradiâncias (22,9 e 66,9 mmol m-2 s-1) em sala de crescimento. O melhor resultado em termos de enraizamento foi obtido no meio de cultura contendo 30 g L-1 de sacarose sob irradiância de 66,9 mmol m-2 s-1. Após a fase de enraizamento in vitro, as plantas foram transferidas para casa de vegetação e mantidas sob nebulização intermitente por 60 dias. Os resultados de sobrevivência aos 30 e 60 dias não diferiram estatisticamente para os dois níveis de irradiância testados. Entretanto, para concentrações de sacarose, a testemunha apresentou sobrevivência inferior (50%) diferindo estatisticamente dos demais tratamentos que foram semelhantes entre si (95 a 100%).
Ornamental pineapple (Ananas bracteatus Schult. f.) is often used in landscape compositions in order to limit areas or borders. Micropropagation allows high multiplication rates and may be an interesting technique of vegetative propagation in order to meet the need of plants of this species that is rising on national and international markets. The objective of this study was to determine the effect of several light irradiances and sucrose concentrations during in vitro rooting on survival during the acclimatization stage. Several concentrations of sucrose (0, 10, 20 or 30 g L-1) in the rooting medium and two light irradiances (22.9 and 66.9 mmol m-2 s-1) in the culture room were tested. The best results of rooting were obtained on culture medium containing 30 g L-1 sucrose under an irradiance of 66.9 mmol m-2 s-1 . The plants were then transferred to the greenhouse and cultured under intermittent mist for 60 days. There was no significant difference among the results of survival under the two irradiances tested. However, for sucrose concentrations, the survival rate of the control (50%) was statistically inferior to that of the other treatments that were similar among them (95 and 100%).
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oliveira2010cinciapr-aclimatizao Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Yohana de Oliveira;Justina Inês Anselmini;Francine Lorena Cuquel;Fernanda Pinto;Marguerite Quoirin
Journal information and software technology
Year 2010
DOI
10.1590/S1413-70542010000700010
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000700010
Keywords
micropropagation micropropagação

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