determination of cilostazol and its active metabolite 3,4-dehydro cilostazol from small plasma volume by uplc−ms/ms

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ID: 243144
2015
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Abstract
A simple, rapid and sensitive ultra performance liquid chromatography-tandem mass spectrometry (UPLC−MS/MS) method has been developed for the simultaneous determination of cilostazol and its pharmacologically active metabolite 3,4-dehydro cilostazol in human plasma using deuterated analogs as internal standards (ISs). Plasma samples were prepared using solid phase extraction and chromatographic separation was performed on UPLC BEH C18 (50 mm×2.1 mm, 1.7 µm) column. The method was established over a concentration range of 0.5–1000 ng/mL for cilostazol and 0.5–500 ng/mL for 3,4-dehydro cilostazol. Intra- and inter-batch precision (% CV) and accuracy for the analytes were found within 0.93–1.88 and 98.8–101.7% for cilostazol and 0.91–2.79 and 98.0–102.7% for the metabolite respectively. The assay recovery was within 95–97% for both the analytes and internal standards. The method was successfully applied to support a bioequivalence study of 100 mg cilostazol in 30 healthy subjects. Keywords: Cilostazol, 3,4-dehydro cilostazol, UPLC−MS/MS, Sensitive, High throughput
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bhatt2015journaldetermination Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Nejal M. Bhatt;Vijay D. Chavada;Daxesh P. Patel;Primal Sharma;Mallika Sanyal;Pranav S. Shrivastav
Journal Frontiers in plant science
Year 2015
DOI
DOI not found
URL
http://www.sciencedirect.com/science/article/pii/S2095177914000689
Keywords
therapeutics pharmacology

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