diversidade de phytophthora parasitica isolados de citrus usando seqüências de nucleotídeos da região its-5.8s rdna genotypic diversity among p. parasitica isolates reveled by its-5.8s rdna nucleotide sequences

Clicks: 287
ID: 242838
2006
Free PDF
Article Quality & Performance Metrics
Overall Quality
Not rated
Combines reader engagement with the AI quality analysis. This article has not been analysed, so there is no overall score — reader engagement is measured and shown alongside.
AI Quality Assessment
Not analyzed
Readership in this journal
Popular

Ranked #11 of 52 articles by views in research on chemical intermediates

Most read Least read

Bar heights use a square-root scale.

Mint this article as an NFT
Not yet minted

Create a permanent, verifiable on-chain record of this article on the Scimatic Network. The NFT is held in your Journament account, and you can withdraw it to your own wallet at any time.

5 SUSD one-off · no wallet required
Sign in to mint
Abstract
Realizou-se estudo para caracterização e verificação da diversidade genética de Phytophthora parasitica, agente causador da gomose dos citros. Quatorze isolados de Phytophthora parasitica, provenientes do Estado de São Paulo, foram seqüenciados a partir das regiões internas transcritas (ITS1 e ITS2) do gene 5.8S. Obtiveram-se seqüências de 812 pb a 860 pb que foram comparadas com seqüências de outras espécies de Phytophthora spp depositadas no NCBI. Foram feitos estudos filogenéticos, utilizando-se o método "neighbor-joining" com 1000 "bootstrap" e construído o dendrograma mais representativo. Obtiveram-se os resultados de 98,88% a 100% de similaridade genética entre os 14 isolados paulistas, e 99,5% a 98,8% entre estes e a seqüência de P. nicotianae (gi| 8927482) obtida do GenBank NCBI.
Phytophthoraparasitica is been considered one of the most prevalent citrus soilborn pathogens in Brazil. Fourteen isolates were collected in different region of São Paulo state. Amplification of the ITS region using primers ITS1 and ITS2 produced DNA fragments of 812 to 860 bp, that were compared with other sequences obtained to Gene Bank (NCBI). Neighbor-joining analysis, with 1000 bootstrap revealed an average similarity of 99,3 to 100% between the isolates, and 98,1 to 99,9% between S. Paulo state isolates and P. nicotianae (= Phytophthoraparasitica)obtained from the Gene Bank (gi|8927482). The ITS1 was more conserved than to the ITS2. This is a good tool for quick diagnosis of Phytophthora citrus diseases.
Reference Key
rosa2006summadiversidade Use this key to autocite in the manuscript while using SciMatic Manuscript Manager or Thesis Manager
Authors ;Daniel Dias Rosa;Marcos Antonio Machado;Maria Luisa Penteado Natividade Targon;Edson Luiz Furtado
Journal research on chemical intermediates
Year 2006
DOI
10.1590/S0100-54052006000200017
URL
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-54052006000200017
Keywords
ITS phylogenetic

Citations

No citations found. To add a citation, contact the admin at info@scimatic.org

Comments

No comments yet. Be the first to comment on this article.