membran spermatozoa hasil seksing gradien albumin berpengencer andromed dan cauda epididymal plasma-2 ditambahkan kuning telur (membrane of sperm following gradient albumin sexing using andromed and cep-2 supplemented with egg yolk)
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ID: 242699
2013
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Abstract
The objective of this study was to determine sperm membrane stability after sexing with gradientalbumin (egg white) using andromed and CEP-2 supplemented with 10% egg yolk. The observations ofsperm membrane included membrane integrity, uncapacitated, capacitated and acrosome reacted. Albumingradient was made by mixing egg white with andromed or CEP-2 supplemented with 10% egg yolk resultingin a concentration of egg whites 10%, 30% and 50%. Membrane integrity was observed using HOST(Hypoosmotic Swelling Test), while uncapacitated, capacitated and acrosome reacted sperm were observedusing CTC (Chlortetracycline) staining. The results showed that both andromed and CEP-2 supplementedwith 10% egg yolk could protect membrane integrity, uncapacitated, capacitated and acrosome reactedsperm. Andromed and CEP-2 supplement with 10% egg yolk could reduce the demage of membrane integrityand uncapacitated sperm, whereas capacitated and acrosome reacted sperm could be kept low.
| Reference Key |
purwoistri2013jurnalmembran
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|---|---|
| Authors | ;Rita Fitria Purwoistri;Trinil Susilawati;Sri Rahayu |
| Journal | journal of advanced research |
| Year | 2013 |
| DOI |
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| Keywords | Keywords not found |
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